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Team:Alberta/References/Publications/Markerless gene replacement in Escherichia coli stimulated by double strand break in the chromosome
From 2009.igem.org
Gyorgy Posfai, Vitaliy Kolisnychenko, Zsuzsa Bereczki and Frederick R. Blattner
Nucleic Acids Research, Vol 27, Issue 22 4409-4415, Copyright © 1999 by Oxford University Press
Abstract: Driven by the needs of functional genomics, DNA engineering by homologous
recombination in Escherichia coli has emerged as a major addition to existing
technologies. Two alternative approaches, RecA-dependent engineering and
ET recombination, allow a wide variety of DNA modifications, including some
which are virtually impossible by conventional methods. These approaches do
not rely on the presence of suitable restriction sites and can be used to insert,
delete or substitute DNA sequences at any desired position on a target
molecule. Furthermore, ET recombination can be used for direct subcloning
and cloning of DNA sequences from complex mixtures, including bacterial
artificial chromosomes and genomic DNA preparations. The strategies
reviewed in this article are applicable to modification of DNA molecules of any
size, including very large ones, and present powerful new avenues for DNA
manipulation in general.
Link: [http://nar.oxfordjournals.org/cgi/content/abstract/27/22/4409 Nucleic Acids Research]
